ABSTRACT
<p><b>OBJECTIVE</b>To study on the chemical consitituents of Lagotis brevituba.</p><p><b>METHOD</b>The chemical consitituents were isolated by silica gel column chromatography, polyamide column chromatography and semi-preparative HPLC, and their structures were identified by spectroscopic methods.</p><p><b>RESULT</b>Eight compounds were isolated and they were identified as beta-sitosterol (1), succinic acid (2), luteolin-7-O-beta-D-glucoside (3), uracil (4), apigenin (5), chrysoeriol (6), chrysoeriol-7-O-beta-D-glucoside (7), and apigenin-7-O-beta-D-glucoside (8).</p><p><b>CONCLUSION</b>Compound 4-8 were isolated from L. brevituba for the first time, and among them, compound 7 and 8 were isolated from genus Lagotis for the first time.</p>
Subject(s)
Chromatography, High Pressure Liquid , Organic Chemicals , Plantago , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Lagotis brevituba.</p><p><b>METHOD</b>The chemical constituents were isolated and purified by silica gel column chromatography, polyamide column chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of analysis of IR,NMR, 2D-NMR, and MS spectra.</p><p><b>RESULT</b>Two compounds were obtained and were identified as phenylethanoid glucosides, lagotiside A (1) and acteoside (2), respectively.</p><p><b>CONCLUSION</b>Compound 1 is a new compound and named as lagotiside A.</p>
Subject(s)
Glucosides , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Extracts , Chemistry , Plantago , ChemistryABSTRACT
Object To determine the contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in the stems and fruits of Schisandra rudriflora (Franch ) Rehd. et Wils , which were collected from different areas Methods An HPLC method was set up: Column, Sphereclone, ODS (250 mm?4 6 mm, 5 ?m); mobil phase, A: H 2O, B: MeOH; gradient elution was with 70% B from 0-4 min, 70%-100% B from 4-54 min; the flow rate was 0 4 mL/min; the column temperature was 25 ℃ and the DAD detector was used at 254 nm Results The contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in S. rubriflora were 0.026%-0.083%, 0.007%-0.945%, 0.002%-0.121%, 0.010%-0.038%, respectively. Deoxyschizandrin exists widely in S. rubriflora and its content is higher in fruits than that in stems Conclusion Deoxyschizandrin is the main active lignan in the fruits of S rubriflora
ABSTRACT
Object To provide a basis for the identification of the crude drug of Fructus Schisandrae Sphenantherae Methods Morpholgical characters and microstructural features of the seed surface of Schisandra sphenanthera Rehd. et Wils , S. rubriflora (Franch ) Rehd. et Wils., S. viridis A. C. Smith, and S. henryi Clarke were observed under light microscope and scanning electron microscope. Identification of them was also completed by TLC qualitative analysis for deoxyschisandrin and schisantherin A Results The fruits of S. sphenanthera, S. rubriflora, S. viridis and S. henryi could be classified to three types with the characters of micromorphological features of the seed surface. The results of TLC showed that deoxyschisandrin and schisantherin A were present in the fruits of S. sphenanthera from Pingli, Shaanxi, Luanchuan, Henan, Yangcheng, Shanxi, S. rubriflora and S.viridis, Hengshan Hunan Provinces The fruit of S. henryi contained a little quantity of schisantherin A, but the fruits of S. sphenanthera from Liuba Shaanxi and Xiaolongshan Gansu Provinces did not have deoxyschisandrin and schisantherin A. Conclusion The fruits of S. sphenanthera from different geographical origin, S. rubriflora, S. viridis and S. henryi can be identified by the characters of micromorphological featrues of the seed surface and the TLC qualitative analysis for deoxyschisandrin and schisantherin A